Vaccinia virus (VACV), a member of the Poxviridae family, uses cytoplasmic factories for its replication. Recent studies indicated that VACV infection requires a set of nucleoporins. However, how the nucleoporins contribute to viral life cycle remains unclear. Here, we report that the nucleoporins Nup62 and Nup358 localize to the cytoplasmic viral factories (VFs). Nup358 was targeted to the VFs at 6h post-infection (hpi), whereas Nup62, along with the previously reported translation factors such as eIF4E, eIF3η and G3BP1, was recruited to the VFs at 8 hpi. Nup358 depletion led to a decrease in the size and number of viral factories and reduction in viral yield. Further studies showed that Nup358 is involved in recruiting Nup62 and eIF4E to the VFs. Collectively, our results reveal spatio-temporal regulation in the recruitment of nucleoporins and translation factors to VFs, and particularly the importance of Nup358 in VACV infection.
Journal article
2017-12-01T00:00:00+00:00
512
151 - 160
9
National Centre for Cell Science, S.P. Pune University Campus, Ganeshkhind, Pune 411007, India.
Cell Line, Hela Cells, Humans, Vaccinia virus, Nuclear Pore Complex Proteins, Eukaryotic Initiation Factor-4E, Molecular Chaperones, DNA, Viral, Virus Replication, Gene Expression Regulation, Protein Transport