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ATAC-Seq Analysis

Examples of different QC metrics from in-house ATAC-Seq data.Examples of different QC metrics from in-house ATAC-Seq data.ATAC-Seq is a relatively new technique that enables the study of chromatin accessibility using a faster approach than DNAse-Seq. It aims to identify DNaseI hypersensitive sites (i.e. all DNA-accessible regions) to determine DNA-binding proteins' "footprints" and to infer nucleosome positions. ATAC-Seq can be performed either on bulk samples or on single cells.

The following files can be provided with ATAC-Seq projects:

  • Primary QC report (general QC metrics on sequencing quality for each sequenced sample
  • FASTQ (raw sequence data) and BAM (alignment) files for each sample

Bespoke downstream ATAC-Seq analyses*:

  • Secondary QC report (a set of QC metrics including fragment size distribution, reproducibility of the experiment and genomic features enrichment)
  • Full peak annotation (including nearest gene annotation and overlap with Encode's TFs, DNaseI and other tracks of interest)
  • Customised ATAC-Seq downstream analyses

* Please note that bespoke analyses might be subject to availability and resources, please contact us for more information.