Fowler Group

The Fowler lab is designing new models of tauopathy that display robust fibrillar and oligomeric tau inclusions to better model tauopathies. We are using these new tools to perform an unbiased interrogation of the subcellular localisation and interactome of proteins and transcripts in CNS cell types to gain an understanding of how and why they succumb to tau pathology.

The Fowler Lab hosts a collection of scientists that merge disparate skill sets to collaborate on a common goal of producing and characterising a set of robust tauopathy models in human iPSCs. Combining AI mutation prediction with CRISPR Cas9 editing has enabled us to produce a panel of i3Neuron lines with unique tau properties. We are currently using these lines to model the changes associated with the induction of tau pathology using highly-resolved subcellular fractionation techniques coupled with proteomic and transcriptomic profiling. We have also optimised specialised techniques for organelle and extracellular vesicle (EV) purification from i3Neurons, and are interested in exploring compartment-specific interactomes for neurodegenerative targets. Our longer-term goals are to identify new targets that will result in drugs with meaningful clinical benefit across various forms of dementia. We are also interested in conformational antibody design, in using circulating EVs as disease biomarkers, and in "seeing beyond" with super-resolution microscopy! Our team is always happy to discuss collaboration - please get in touch!