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Chromosome Dynamics & Microscopy Services and Cost Recovery Charges

1. Chromosome Dynamics Core

The Chromosome Dynamics service offered by the Core consists of a range of different Fluorescence in situ Hybridization (FISH) applications including:

  • Metaphase and Interphase FISH (chromosomal assignment and clone ordering)
  • Fibre-FISH (high resolution mapping
  • Chromosome Painting
  • M-FISH (multicolour karyotyping)
  • RNA-FISH (cell-based gene expression assay)
  • FISH on paraffin sections (analysis of archive material)
  • ImmunoFISH (combined FISH and Immunofluorescence)
  • Copy number variation (Affymetrix GeneChip Cytogenetics Array system).

Related Tissue Culture Techniques:

  • Revival and maintenance of transformed cell lines
  • Establishment of primary cell cultures
  • Chromosome preparations from cell lines, blood cultures and fibroblast cultures

Probe Preparation:

  • DNA mini-preps
  • DNA labeling by nick-translation

The Core group can also provide support with the following cytological techniques:

  • Immunofluorescence
  • Chromogenic RNA in situ hybridization (ISH) - The Cellular Imaging Core maintains a Leica CM-3050-S cryostat for histological sectioning

Additional

  • General consultation and pre-application advice on research projects involving Molecular Cytogenetics and other immunofluorescence cytological techniques
  • Hands-on training in Fluorescence in situ Hybridization (FISH) and immunoctytochemistry

2. Microscopy

As part of the Microscopy Facilities made available by the Cellular Imaging Core, the Chromosome Dynamics laboratory houses the following fluorescence microscope for providing Cores services within the Centre:

  • An Olympus BX-51 upright wide-field microscope with a PC workstation running Leica Cytovision software for imaging samples on glass slides, suitable for FISH, M-FISH and chromosome analysis

Our Cost Recovery Charges

We operate a cost recovery system for the molecular cytogenetic services we offer, although this is a minimal charge and only recovers the cost of the consumables we have to use for the techniques.

Download our cost recovery charges as an Adobe pdf file. You may also download our Core flyer detailing the services we offer.

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